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UGCG ELISA 试剂盒

UGCG 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674820
发货至: 中国
  • 抗原 See all UGCG ELISA试剂盒
    UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))
    适用
    • 3
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0115 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all UGCG ELISA试剂盒
    UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))
    别名
    UDP Glucose Ceramide Glucosyltransferase (UGCG) (UGCG 产品)
    别名
    GCS ELISA Kit, GLCT1 ELISA Kit, XLCGT ELISA Kit, gcs ELISA Kit, glct1 ELISA Kit, ugcga ELISA Kit, xlcgt ELISA Kit, cb539 ELISA Kit, sb:cb539 ELISA Kit, zgc:112506 ELISA Kit, ugcg ELISA Kit, ugcgb ELISA Kit, AU043821 ELISA Kit, C80537 ELISA Kit, Epcs21 ELISA Kit, GlcT-1 ELISA Kit, Ugcgl ELISA Kit, UDP-glucose ceramide glucosyltransferase ELISA Kit, UDP-glucose ceramide glucosyltransferase L homeolog ELISA Kit, UDP-glucose ceramide glucosyltransferase S homeolog ELISA Kit, UGCG ELISA Kit, ugcg.L ELISA Kit, Ugcg ELISA Kit, ugcg ELISA Kit, ugcg.S ELISA Kit
    背景
    Alternative name: GCS, GLCT-1, Glucosylceramide Synthase, N-acylsphingosine D-glucosyltransferase
    基因ID
    7357
    UniProt
    Q16739
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