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CAT2 ELISA 试剂盒

This anti-CAT2 antibody (ABIN5674785) is a antibody detecting CAT2 in ELISA. Suitable for Human.
产品编号 ABIN5674785
发货至: 中国

Quick Overview for CAT2 ELISA 试剂盒 (ABIN5674785)

抗原

See all CAT2 products
CAT2 (Cationic Amino Acid Transporter 2 (CAT2))

适用

  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.156 ng/mL - 10 ng/mL

应用范围

ELISA

样品类型

Tissue Homogenate
  • 最低检测浓度

    0.156 ng/mL

    Analytical Method

    Quantitative

    灵敏度

    0054 ng/mL

    组件

    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual

    试剂未包括

    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    实验时间

    1 - 4.5 h

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    有效期

    12 months
  • 抗原 See all CAT2 products

    CAT2 (Cationic Amino Acid Transporter 2 (CAT2))

    背景

    Alternative name: SLC7A2, ATRC2, CAT-2, HCAT2, Low Affinity Cationic Amino Acid Transporter 2, Solute Carrier Family 7 Member 2,Cationic Amino Acid Transporter,y+ System

    基因ID

    6542

    UniProt

    P52569
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