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AVPR1B ELISA 试剂盒

AVPR1B 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Tissue Homogenate
产品编号 ABIN5674780
发货至: 中国
  • 抗原 See all AVPR1B ELISA试剂盒
    AVPR1B (Arginine Vasopressin Receptor 1B (AVPR1B))
    适用
    • 1
    • 1
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.625 ng/mL - 40 ng/mL
    最低检测浓度
    0.625 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0266 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all AVPR1B ELISA试剂盒
    AVPR1B (Arginine Vasopressin Receptor 1B (AVPR1B))
    Abstract
    AVPR1B 产品
    别名
    LOC443050 ELISA Kit, AVPR1B ELISA Kit, si:ch211-125a15.3 ELISA Kit, AVPR3 ELISA Kit, V3/V1b ELISA Kit, VIBR ELISA Kit, VPR3 ELISA Kit, arginine vasopressin receptor 1B ELISA Kit, arginine vasopressin receptor 1Ab ELISA Kit, AVPR1B ELISA Kit, avpr1ab ELISA Kit, Avpr1b ELISA Kit
    背景
    Alternative name: AVPR1-B, AVPR3, Vasopressin 3 Receptor, Antidiuretic Hormone Receptor 1b
    基因ID
    29462
    UniProt
    P48974
    途径
    Regulation of Systemic Arterial Blood Pressure by Hormones
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