beta Actin ELISA 试剂盒
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- 抗原 See all beta Actin (ACTB) ELISA试剂盒
- beta Actin (ACTB) (Actin, beta (ACTB))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0.69 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Actin, beta (ACTB) ELISA Kit
ACTB 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Actin, beta (ACTB) ELISA KitACTB 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Actin, beta (ACTB) ELISA KitACTB 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Actin, beta (ACTB) ELISA KitACTB 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Actin, beta (ACTB) ELISA KitACTB 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all beta Actin (ACTB) ELISA试剂盒
- beta Actin (ACTB) (Actin, beta (ACTB))
- 别名
- Actin Beta (ACTb) (ACTB 产品)
- 别名
- BRWS1 ELISA Kit, PS1TP5BP1 ELISA Kit, ACTA1 ELISA Kit, Actx ELISA Kit, E430023M04Rik ELISA Kit, beta-actin ELISA Kit, ps1tp5bp1 ELISA Kit, act-2 ELISA Kit, actb ELISA Kit, B-actin ELISA Kit, LOC100174883 ELISA Kit, LOC100304816 ELISA Kit, A ELISA Kit, ACT5C ELISA Kit, Ac5C ELISA Kit, Act ELISA Kit, Act-5C ELISA Kit, Act5 ELISA Kit, Act5c ELISA Kit, Actin ELISA Kit, Actin/BAP47 ELISA Kit, Actin5C ELISA Kit, BAP47 ELISA Kit, Bap47 ELISA Kit, CG4027 ELISA Kit, Dmel\\CG4027 ELISA Kit, M32055 ELISA Kit, T11 ELISA Kit, act ELISA Kit, act 5C ELISA Kit, act5C ELISA Kit, actin ELISA Kit, actin5C ELISA Kit, anon-EST:fe2D2 ELISA Kit, beta-actin/Bap47 ELISA Kit, cyt5C ELISA Kit, l(1)G0009 ELISA Kit, l(1)G0010 ELISA Kit, l(1)G0025 ELISA Kit, l(1)G0079 ELISA Kit, l(1)G0117 ELISA Kit, l(1)G0177 ELISA Kit, l(1)G0245 ELISA Kit, l(1)G0330 ELISA Kit, l(1)G0420 ELISA Kit, l(1)G0486 ELISA Kit, Bact ELISA Kit, ACT-5 ELISA Kit, ACTB ELISA Kit, B-ACTZF ELISA Kit, actba ELISA Kit, bact ELISA Kit, bactin1 ELISA Kit, bactzf ELISA Kit, wu:fd18f05 ELISA Kit, A26C1B ELISA Kit, POTE2alpha ELISA Kit, POTEACTIN ELISA Kit, Beta-actin ELISA Kit, actin beta ELISA Kit, actin, beta ELISA Kit, cytoplasmic actin OlCA1 ELISA Kit, beta-actin ELISA Kit, Actin 5C ELISA Kit, actin gamma 1 ELISA Kit, actin, beta 1 ELISA Kit, actin, beta L homeolog ELISA Kit, beta actin ELISA Kit, POTE ankyrin domain family member F ELISA Kit, actin, beta like 2 ELISA Kit, actin, cytoplasmic 1 ELISA Kit, ACTB ELISA Kit, Actb ELISA Kit, actb ELISA Kit, LOC100174883 ELISA Kit, LOC100304816 ELISA Kit, Act5C ELISA Kit, ACTG1 ELISA Kit, actb1 ELISA Kit, actb.L ELISA Kit, LOC100136352 ELISA Kit, POTEF ELISA Kit, ACTBL2 ELISA Kit, LOC109073280 ELISA Kit
- 背景
- Alternative name: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
- 基因ID
- 11461
- UniProt
- P60710
- 途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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