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GBA ELISA 试剂盒

GBA 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5674742
发货至: 中国
  • 抗原 See all GBA ELISA试剂盒
    GBA (Glucosidase, Beta, Acid (GBA))
    适用
    • 2
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0064 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all GBA ELISA试剂盒
    GBA (Glucosidase, Beta, Acid (GBA))
    别名
    Glucosidase Beta, Acid (GbA) (GBA 产品)
    别名
    GBA1 ELISA Kit, GCB ELISA Kit, GLUC ELISA Kit, BETA-GLUCOSIDASE ELISA Kit, T12J13.8 ELISA Kit, T12J13_8 ELISA Kit, beta glucosidase 25 ELISA Kit, PSPTO3318 ELISA Kit, PSPTO4290 ELISA Kit, GC ELISA Kit, GCase ELISA Kit, betaGC ELISA Kit, glucosylceramidase beta ELISA Kit, beta glucosidase 25 ELISA Kit, beta-glucosidase ELISA Kit, Beta-glucosidase ELISA Kit, glucosidase, beta, acid ELISA Kit, glucosylceramidase ELISA Kit, bglX-2 ELISA Kit, GBA ELISA Kit, BGLU25 ELISA Kit, PSPTO_3318 ELISA Kit, bglX ELISA Kit, bglA4 ELISA Kit, Gba ELISA Kit, LOC100399524 ELISA Kit, bglA.2 ELISA Kit, bglX-2 ELISA Kit
    背景
    Alternative name: GBA1, GCB, GLUC, Imiglucerase, Alglucerase, Beta-glucocerebrosidase, Glucosylceramidase, β-Glucosidase, D-Glucosyl-N-Acylsphingosine Glucohydrolase
    基因ID
    14466
    UniProt
    P17439
    途径
    Cellular Glucan Metabolic Process
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