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VLDLR ELISA 试剂盒

VLDLR 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
产品编号 ABIN5674735
发货至: 中国
  • 抗原 See all VLDLR ELISA试剂盒
    VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.56 ng/mL - 100 ng/mL
    最低检测浓度
    1.56 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0.53 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all VLDLR ELISA试剂盒
    VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
    Abstract
    VLDLR 产品
    别名
    CARMQ1 ELISA Kit, CHRMQ1 ELISA Kit, VLDLRCH ELISA Kit, AA408956 ELISA Kit, AI451093 ELISA Kit, AW047288 ELISA Kit, vldlr ELISA Kit, very low density lipoprotein receptor ELISA Kit, very low density lipoprotein receptor S homeolog ELISA Kit, VLDLR ELISA Kit, Vldlr ELISA Kit, vldlr.S ELISA Kit
    背景
    Alternative name: VLDL-R
    基因ID
    22359
    UniProt
    P98156
    途径
    Cellular Response to Molecule of Bacterial Origin
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