ADCY10 ELISA 试剂盒
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- 抗原 See all ADCY10 ELISA试剂盒
- ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0051 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Adenylate Cyclase 10 (Soluble) (ADCY10) ELISA Kit
ADCY10 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase 10 (Soluble) (ADCY10) ELISA KitADCY10 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase 10 (Soluble) (ADCY10) ELISA KitADCY10 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
Adenylate Cyclase 10 (Soluble) (ADCY10) ELISA KitADCY10 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all ADCY10 ELISA试剂盒
- ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
- 别名
- Adenylate Cyclase 10, Soluble (ADCY10) (ADCY10 产品)
- 别名
- HCA2 ELISA Kit, RP1-313L4.2 ELISA Kit, SAC ELISA Kit, SACI ELISA Kit, Sacy ELISA Kit, Sac ELISA Kit, 4930431D04Rik ELISA Kit, 4931412F17 ELISA Kit, sAC ELISA Kit, ADCY10 ELISA Kit, SACY ELISA Kit, adenylate cyclase 10 ELISA Kit, adenylate cyclase 10 (soluble) ELISA Kit, ADCY10 ELISA Kit, Adcy10 ELISA Kit
- 背景
- Alternative name: SAC, HCA2, SACI, Sacy, Testicular soluble adenylyl cyclase, Hypercalciuria,Absorptive 2, AH-related protein, Germ cell soluble adenylyl cyclase
- 基因ID
- 55811
- UniProt
- Q96PN6
- 途径
- cAMP Metabolic Process
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