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MAT1A ELISA 试剂盒

MAT1A 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Tissue Homogenate
产品编号 ABIN5674721
发货至: 中国
  • 抗原 See all MAT1A ELISA试剂盒
    MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))
    适用
    • 3
    • 3
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.625 ng/mL - 40 ng/mL
    最低检测浓度
    0.625 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0255 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all MAT1A ELISA试剂盒
    MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))
    别名
    Methionine Adenosyltransferase I Alpha (MAT1a) (MAT1A 产品)
    别名
    MAT ELISA Kit, MATA1 ELISA Kit, SAMS ELISA Kit, SAMS1 ELISA Kit, AdoMet ELISA Kit, SADE ELISA Kit, SAS ELISA Kit, AI046368 ELISA Kit, Ams ELISA Kit, wu:fi35e01 ELISA Kit, zgc:55442 ELISA Kit, methionine adenosyltransferase 1A ELISA Kit, methionine adenosyltransferase I, alpha ELISA Kit, MAT1A ELISA Kit, Mat1a ELISA Kit, mat1a ELISA Kit
    背景
    Alternative name: MAT, SAMS, MATA1, SAMS1, AMS1, S-Adenosylmethionine Synthetase, S-adenosylmethionine synthase isoform type-1
    基因ID
    4143
    UniProt
    Q00266
    途径
    Mitotic G1-G1/S Phases, M Phase, Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
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