Podocan ELISA 试剂盒
-
- 抗原 See all Podocan (PODN) ELISA试剂盒
- Podocan (PODN)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.125 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.125 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 26.5 pg/mL
- 组件
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
-
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling PODN ELISA Kit
- Top Product
- Discover our top product PODN ELISA Kit
-
Podocan (PODN) ELISA Kit
PODN 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Podocan (PODN) ELISA KitPODN 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all Podocan (PODN) ELISA试剂盒
- Podocan (PODN)
- Abstract
- PODN 产品
- 别名
- LRRGT00160 ELISA Kit, PODN ELISA Kit, PCAN ELISA Kit, SLRR5A ELISA Kit, 9430070G18 ELISA Kit, Pcan ELISA Kit, podocan ELISA Kit, Podn ELISA Kit, PODN ELISA Kit, podn ELISA Kit
- 背景
- Alternative name: PCAN, SLRR5A
- 基因ID
- 127435
- UniProt
- Q7Z5L7
-
