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OPTN ELISA 试剂盒

OPTN 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Tissue Homogenate
产品编号 ABIN5674718
发货至: 中国
  • 抗原 See all OPTN ELISA试剂盒
    OPTN (Optineurin (OPTN))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78.125 pg/mL - 5000 pg/mL
    最低检测浓度
    78.125 pg/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    32.4 pg/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product OPTN ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all OPTN ELISA试剂盒
    OPTN (Optineurin (OPTN))
    Abstract
    OPTN 产品
    别名
    nrp ELISA Kit, fip2 ELISA Kit, hip7 ELISA Kit, hypl ELISA Kit, glc1e ELISA Kit, tfiiia-intp ELISA Kit, ALS12 ELISA Kit, FIP2 ELISA Kit, GLC1E ELISA Kit, HIP7 ELISA Kit, HYPL ELISA Kit, NRP ELISA Kit, TFIIIA-INTP ELISA Kit, Fip2 ELISA Kit, 4930441O07Rik ELISA Kit, fj52f04 ELISA Kit, si:ch211-240l19.3 ELISA Kit, wu:fj52f04 ELISA Kit, zgc:66386 ELISA Kit, zgc:77868 ELISA Kit, optineurin ELISA Kit, optineurin L homeolog ELISA Kit, OPTN ELISA Kit, optn ELISA Kit, Optn ELISA Kit, optn.L ELISA Kit
    背景
    Alternative name: NRP, FIP2, GLC1E, HIP7, HYPL, TFIIIA-INTP, Glaucoma 1,Open Angle,E(Adult-Onset), Huntingtin-interacting protein 7, Optic neuropathy-inducing protein, E3-14.7K-interacting protein
    基因ID
    246294
    UniProt
    Q8R5M4
    途径
    M Phase
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