Nodal ELISA 试剂盒
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- 抗原 See all Nodal (NODAL) ELISA试剂盒
- Nodal (NODAL) (Nodal Homolog (NODAL))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.625 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.625 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 6.0 pg/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Nodal Homolog (NODAL) ELISA Kit
NODAL 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nodal Homolog (NODAL) ELISA KitNODAL 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all Nodal (NODAL) ELISA试剂盒
- Nodal (NODAL) (Nodal Homolog (NODAL))
- Abstract
- NODAL 产品
- 别名
- NODAL ELISA Kit, Tg.413d ELISA Kit, HTX5 ELISA Kit, nodal4 ELISA Kit, nodal4-A ELISA Kit, nr4 ELISA Kit, xnr4 ELISA Kit, Xnr-1 ELISA Kit, nodal ELISA Kit, nr1 ELISA Kit, nr1-A ELISA Kit, nodal growth differentiation factor ELISA Kit, nodal homolog (mouse) ELISA Kit, nodal ELISA Kit, nodal growth differentiation factor L homeolog ELISA Kit, nodal homolog 1 L homeolog ELISA Kit, Nodal ELISA Kit, NODAL ELISA Kit, nodal.L ELISA Kit, nodal1.L ELISA Kit
- 基因ID
- 4838
- UniProt
- Q96S42
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity
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