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NPY ELISA 试剂盒

High Sensitivity NPY 适用: 小鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5665050
发货至: 中国
  • 抗原 See all NPY ELISA试剂盒
    NPY (Neuropeptide Y (NPY))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    2.47 pg/mL - 200 pg/mL
    最低检测浓度
    2.47 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性

    This assay has high sensitivity and excellent specificity for detection of High Sensitive Neuropeptide Y (NPY).
    No significant cross-reactivity or interference between High Sensitive Neuropeptide Y (NPY) and analogues was observed.

    灵敏度
    0.94 pg/mL
    质量等级
    High Sensitivity
    组件
    • Pre-coated, ready to use 96-well strip plate
    • Plate sealer for 96 wells
    • Standard Diluent
    • Assay Diluent A
    • Assay Diluent B
    • Stop Solution
    • Standard
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    试剂未包括
    • Microplate reader with 450 nm filter.
    • Precision single or multi-channel pipettes and disposable tips.
    • Eppendorf Tubes for diluting samples.
    • Deionized or distilled water.
    • Absorbent paper for blotting the microtiter plate.
    • Container for Wash Solution
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  • 应用备注
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    样本量
    50 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Neuropeptide Y (NPY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Neuropeptide Y (NPY) and unlabeled High Sensitive Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific to High Sensitive Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Neuropeptide Y (NPY) in the sample.
    实验流程
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      And then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Neuropeptide Y (NPY) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Neuropeptide Y (NPY) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制
    仅限研究用
  • 注意事项
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    注意事项
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    储存条件
    4 °C
    储存方法
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    有效期
    6 months
  • 抗原 See all NPY ELISA试剂盒
    NPY (Neuropeptide Y (NPY))
    Abstract
    NPY 产品
    别名
    0710005A05Rik ELISA Kit, PYY4 ELISA Kit, NPY02 ELISA Kit, RATNPY ELISA Kit, RATNPY02 ELISA Kit, si:dkey-22m8.5 ELISA Kit, npy ELISA Kit, npyb ELISA Kit, preproNPY ELISA Kit, pyy4 ELISA Kit, xnpy ELISA Kit, npya ELISA Kit, MGC86288 ELISA Kit, prepronpy ELISA Kit, NPY ELISA Kit, NPY1 ELISA Kit, neuropeptide Y ELISA Kit, neuropeptide Y S homeolog ELISA Kit, neuropeptide Y L homeolog ELISA Kit, Npy ELISA Kit, NPY ELISA Kit, npy ELISA Kit, npy.S ELISA Kit, npy.L ELISA Kit, LOC100533423 ELISA Kit
    途径
    Feeding Behaviour
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