WT1 CLIA Kit
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- 抗原 See all WT1 CLIA Kits
- WT1 (Wilms Tumor 1 (WT1))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.
- 灵敏度
- 6.21 pg/mL
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Wilms Tumor 1 (WT1) ELISA Kit
WT1 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Wilms Tumor 1 (WT1) ELISA KitWT1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wilms Tumor Protein (WT1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all WT1 CLIA Kits
- WT1 (Wilms Tumor 1 (WT1))
- 别名
- Wilms Tumor Protein (WT1 产品)
- 别名
- AWT1 CLIA Kit, EWS-WT1 CLIA Kit, GUD CLIA Kit, NPHS4 CLIA Kit, WAGR CLIA Kit, WIT-2 CLIA Kit, WT33 CLIA Kit, CWT1 CLIA Kit, WT1 CLIA Kit, XWT1a CLIA Kit, wt-1 CLIA Kit, wt1 CLIA Kit, xWT1 CLIA Kit, WT-1 CLIA Kit, gud CLIA Kit, wagr CLIA Kit, wt33 CLIA Kit, xwt1 CLIA Kit, wit-2 CLIA Kit, si:ch211-160j6.3 CLIA Kit, zgc:152697 CLIA Kit, D630046I19Rik CLIA Kit, Wt-1 CLIA Kit, XWT1b CLIA Kit, XeWT1 CLIA Kit, Wilms tumor 1 CLIA Kit, Wilms tumor 1 S homeolog CLIA Kit, wilms tumor 1b CLIA Kit, Wilms tumor protein CLIA Kit, Wilms tumor 1 homolog CLIA Kit, Wilms tumor 1 L homeolog CLIA Kit, WT1 CLIA Kit, wt1.S CLIA Kit, wt1 CLIA Kit, wt1b CLIA Kit, Wt1 CLIA Kit, wt1.L CLIA Kit
- 背景
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Gene Name: Wilms Tumor Protein
Gene Aliases: WAGR, GUD, WIT-2, WT33
- 基因ID
- 7490
- UniProt
- P19544
- 途径
- Tube Formation
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