Vitamin D-Binding Protein CLIA Kit
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- 抗原 See all Vitamin D-Binding Protein (GC) CLIA Kits
- Vitamin D-Binding Protein (GC)
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 3.91 ng/mL - 1000 ng/mL
- 最低检测浓度
- 3.91 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP). No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.
- 灵敏度
- 0.59 ng/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vitamin D Binding Protein (DBP) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Vitamin D-Binding Protein (GC) CLIA Kits
- Vitamin D-Binding Protein (GC)
- 别名
- Vitamin D Binding Protein (GC 产品)
- 别名
- DBP CLIA Kit, DBP/GC CLIA Kit, GRD3 CLIA Kit, VDBG CLIA Kit, VDBP CLIA Kit, zgc:110389 CLIA Kit, zgc:92753 CLIA Kit, GC CLIA Kit, Gc CLIA Kit, DBP02 CLIA Kit, VDB CLIA Kit, Vdbp CLIA Kit, VTDB CLIA Kit, gc CLIA Kit, GC, vitamin D binding protein CLIA Kit, D-box binding PAR bZIP transcription factor CLIA Kit, D site albumin promoter binding protein CLIA Kit, group-specific component (vitamin D binding protein) CLIA Kit, group specific component CLIA Kit, group-specific component (vitamin D binding protein) S homeolog CLIA Kit, GC CLIA Kit, Dbp CLIA Kit, gc CLIA Kit, Gc CLIA Kit, gc.S CLIA Kit
- 背景
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Gene Name: Vitamin D Binding Protein
Gene Aliases: GC, VDBG, VDBP, Group-Specific Component, Gc-globulin
- 基因ID
- 2638
- UniProt
- P02774
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process
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