UCHL1 ELISA 试剂盒
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- 抗原 See all UCHL1 ELISA试剂盒
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) and analogues was observed.
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Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA Kit
UCHL1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitHigh Sensitivity UCHL1 适用: 人 C-Term Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 人 C-Term Colorimetric Sandwich ELISA 6.25-400 ng/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 适用: 大鼠 C-Term Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all UCHL1 ELISA试剂盒
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
- 别名
- Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1 产品)
- 别名
- PARK5 ELISA Kit, PGP 9.5 ELISA Kit, PGP9.5 ELISA Kit, PGP95 ELISA Kit, Uch-L1 ELISA Kit, UCH-L1 ELISA Kit, UCHL1 ELISA Kit, cb358 ELISA Kit, wu:fc55h08 ELISA Kit, park5 ELISA Kit, pgp9.5 ELISA Kit, uch-l1 ELISA Kit, MGC132191 ELISA Kit, uchl1 ELISA Kit, AW822034 ELISA Kit, C88048 ELISA Kit, R75593 ELISA Kit, UCHL-1 ELISA Kit, gad ELISA Kit, ubiquitin C-terminal hydrolase L1 ELISA Kit, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA Kit, ubiquitin C-terminal hydrolase L1 L homeolog ELISA Kit, ubiquitin carboxyl-terminal esterase L1 ELISA Kit, ubiquitin carboxy-terminal hydrolase L1 ELISA Kit, UCHL1 ELISA Kit, uchl1 ELISA Kit, uchl1.L ELISA Kit, LOC100304750 ELISA Kit, Uchl1 ELISA Kit
- 基因ID
- 7345
- UniProt
- P09936
- 途径
- Feeding Behaviour
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