Tyrosine Hydroxylase ELISA 试剂盒
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- Tyrosine Hydroxylase (TH)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH). No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.
- 灵敏度
- 0.062 ng/mL
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Tyrosine Hydroxylase (TH) ELISA Kit
TH 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tyrosine Hydroxylase (TH) ELISA KitTH 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Tyrosine Hydroxylase (TH) ELISA KitTH 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tyrosine Hydroxylase (TH) ELISA KitTH 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tyrosine Hydroxylase (TH) ELISA KitTH 适用: 豚鼠 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine Hydroxylase (TH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine Hydroxylase (TH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine Hydroxylase (TH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine Hydroxylase (TH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Tyrosine Hydroxylase (TH) ELISA试剂盒
- Tyrosine Hydroxylase (TH)
- 别名
- Tyrosine Hydroxylase (TH 产品)
- 别名
- DYT14 ELISA Kit, DYT5b ELISA Kit, TYH ELISA Kit, The ELISA Kit, CG10118 ELISA Kit, DH65B ELISA Kit, DTH ELISA Kit, Dmel\\CG10118 ELISA Kit, Pale ELISA Kit, Ple ELISA Kit, TH ELISA Kit, Th ELISA Kit, VII ELISA Kit, dTH1 ELISA Kit, dTH65B ELISA Kit, pale/ple ELISA Kit, th ELISA Kit, dyt14 ELISA Kit, dyt5b ELISA Kit, tyh ELISA Kit, tyrosine hydroxylase ELISA Kit, pale ELISA Kit, tyrosine 3-monooxygenase ELISA Kit, tyrosine hydroxylase S homeolog ELISA Kit, TH ELISA Kit, Th ELISA Kit, ple ELISA Kit, th ELISA Kit, th.S ELISA Kit, LOC100008895 ELISA Kit
- 背景
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Gene Name: Tyrosine Hydroxylase
Gene Aliases: TYH, DYT5b, Tyrosine 3-Monooxygenase, Dystonia 14
- 途径
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
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