TPI1 ELISA 试剂盒
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- 抗原 See all TPI1 ELISA试剂盒
- TPI1 (Triosephosphate Isomerase 1 (TPI1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.625 ng/mL - 40 ng/mL
- 最低检测浓度
- 0.625 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Triosephosphate Isomerase 1 (TPI1). No significant cross-reactivity or interference between Triosephosphate Isomerase 1 (TPI1) and analogues was observed.
- 灵敏度
- 0.252 ng/mL
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Triosephosphate Isomerase 1 (TPI1) ELISA Kit
TPI1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Triosephosphate Isomerase 1 (TPI1) ELISA KitTPI1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Triosephosphate Isomerase 1 (TPI1) ELISA KitTPI1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Triosephosphate Isomerase 1 (TPI1) ELISA KitTPI1 适用: 人 Colorimetric Sandwich ELISA 28 pg/mL - 1800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triosephosphate Isomerase 1 (TPI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Triosephosphate Isomerase 1 (TPI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Triosephosphate Isomerase 1 (TPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Triosephosphate Isomerase 1 (TPI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triosephosphate Isomerase 1 (TPI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triosephosphate Isomerase 1 (TPI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TPI1 ELISA试剂盒
- TPI1 (Triosephosphate Isomerase 1 (TPI1))
- 别名
- Triosephosphate Isomerase 1 (TPI1 产品)
- 别名
- TIM ELISA Kit, TPI ELISA Kit, TIM1 ELISA Kit, hTIM ELISA Kit, tpi ELISA Kit, tpi1 ELISA Kit, TPI1 ELISA Kit, AI255506 ELISA Kit, Tpi ELISA Kit, Tpi-1 ELISA Kit, GB17473 ELISA Kit, Tim ELISA Kit, CG2171 ELISA Kit, Dmel\\CG2171 ELISA Kit, ND14 ELISA Kit, TPIS ELISA Kit, sgk ELISA Kit, wstd ELISA Kit, DrTPI-A ELISA Kit, DrTPI-B ELISA Kit, TIM-B ELISA Kit, cb179 ELISA Kit, wu:fb51c02 ELISA Kit, triosephosphate isomerase 1 ELISA Kit, timeless circadian regulator ELISA Kit, triosephosphate isomerase 1 S homeolog ELISA Kit, triosephosphate isomerase ELISA Kit, triose-phosphate isomerase ELISA Kit, Triose-phosphate isomerase ELISA Kit, triose-phosphate isomerase, putative ELISA Kit, timeless circadian clock ELISA Kit, Triose phosphate isomerase ELISA Kit, Triosephosphate isomerase ELISA Kit, triosephosphate isomerase 1a ELISA Kit, triosephosphate isomerase 1b ELISA Kit, TPI1 ELISA Kit, TIMELESS ELISA Kit, tpi1 ELISA Kit, Tpi1 ELISA Kit, tpi1.S ELISA Kit, Tpi ELISA Kit, tpiA ELISA Kit, CNE05000 ELISA Kit, Fnod_0738 ELISA Kit, Tlet_0996 ELISA Kit, SARE_RS16215 ELISA Kit, PKH_126640 ELISA Kit, Timeless ELISA Kit, tpi-1 ELISA Kit, tpi1a ELISA Kit, tpi1b ELISA Kit
- 背景
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Gene Name: Triosephosphate Isomerase 1
Gene Aliases: TIM, Triose-phosphate isomerase
- 基因ID
- 7167
- UniProt
- P60174
- 途径
- Cell RedoxHomeostasis
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