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TTR ELISA 试剂盒

TTR 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5659513
发货至: 中国
  • 抗原 See all TTR ELISA试剂盒
    TTR (Transthyretin (TTR))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Transthyretin (TTR). No significant cross-reactivity or interference between Transthyretin (TTR) and analogues was observed.
    灵敏度
    0.071 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transthyretin (TTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transthyretin (TTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transthyretin (TTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transthyretin (TTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transthyretin (TTR) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transthyretin (TTR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all TTR ELISA试剂盒
    TTR (Transthyretin (TTR))
    别名
    Transthyretin (TTR 产品)
    别名
    zgc:103583 ELISA Kit, TTR ELISA Kit, LOC100226992 ELISA Kit, CTS ELISA Kit, CTS1 ELISA Kit, HsT2651 ELISA Kit, PALB ELISA Kit, TBPA ELISA Kit, AA408768 ELISA Kit, AI787086 ELISA Kit, D17860 ELISA Kit, prealbumin ELISA Kit, Lr1 ELISA Kit, Tbpa ELISA Kit, Prealbumin ELISA Kit, xTTR ELISA Kit, transthyretin (prealbumin, amyloidosis type I) ELISA Kit, transthyretin ELISA Kit, Transthyretin ELISA Kit, transthyretin (prealbumin, amyloidosis type 1) ELISA Kit, transthyretin-like ELISA Kit, transthyretin L homeolog ELISA Kit, ttr ELISA Kit, TTR ELISA Kit, vpb38 ELISA Kit, MRAD2831_RS38185 ELISA Kit, Caci_4246 ELISA Kit, LOC100226992 ELISA Kit, Ttr ELISA Kit, ttr.L ELISA Kit
    背景

    Gene Name: Transthyretin

    Gene Aliases: TBPA, PA, PLB, ATTR, PALB, Prealbumin, Prealbumin Amyloidosis Type I, Transports Thyroxine and Retinol

    基因ID
    22139
    UniProt
    P07309
    途径
    Hormone Transport
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