Transglutaminase 2 CLIA Kit
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- 抗原 See all Transglutaminase 2 (TGM2) CLIA Kits
- Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 2.5 ng/mL - 160 ng/mL
- 最低检测浓度
- 2.5 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Transglutaminase 2, Tissue (TGM2). No significant cross-reactivity or interference between Transglutaminase 2, Tissue (TGM2) and analogues was observed.
- 灵敏度
- 0.11 ng/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 2, Tissue (TGM2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transglutaminase 2, Tissue (TGM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transglutaminase 2, Tissue (TGM2) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 2, Tissue (TGM2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 2, Tissue (TGM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Transglutaminase 2 (TGM2) CLIA Kits
- Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))
- 别名
- Transglutaminase 2, Tissue (TGM2 产品)
- 别名
- G-ALPHA-h CLIA Kit, GNAH CLIA Kit, TG2 CLIA Kit, TGC CLIA Kit, tgm2 CLIA Kit, zgc:101081 CLIA Kit, TgaseII CLIA Kit, TGM2 CLIA Kit, fc12b04 CLIA Kit, wu:fc12b04 CLIA Kit, zgc:136700 CLIA Kit, TGL2 CLIA Kit, G[a]h CLIA Kit, TGase2 CLIA Kit, tTG CLIA Kit, tTGas CLIA Kit, transglutaminase 2 CLIA Kit, transglutaminase 2, C polypeptide A CLIA Kit, transglutaminase 2b CLIA Kit, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) CLIA Kit, transglutaminase 2, C polypeptide CLIA Kit, TGM2 CLIA Kit, tgm2a CLIA Kit, Tgm2 CLIA Kit, tgm2b CLIA Kit
- 背景
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Gene Name: Transglutaminase 2, Tissue
Gene Aliases: tTG, TG2, C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase, Transglutaminase C, Transglutaminase H
- 基因ID
- 7052
- UniProt
- P21980
- 途径
- Tube Formation, Thromboxane A2 Receptor Signaling
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