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Thymosin beta 10 ELISA 试剂盒

TMSB10 适用: 人 Colorimetric Competition ELISA 24.69 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5659215
发货至: 中国
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    Thymosin beta 10 (TMSB10)
    适用
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    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    24.69 ng/mL - 2000 ng/mL
    最低检测浓度
    24.69 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Thymosin Beta 10 (TMSb10). No significant cross-reactivity or interference between Thymosin Beta 10 (TMSb10) and analogues was observed.
    灵敏度
    13.52 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 h
    板类型
    Pre-coated
    实验流程
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymosin Beta 10 (TMSb10) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymosin Beta 10 (TMSb10) and unlabeled Thymosin Beta 10 (TMSb10) (Standards or samples) with the pre-coated antibody specific to Thymosin Beta 10 (TMSb10). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymosin Beta 10 (TMSb10) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymosin Beta 10 (TMSb10) in the sample.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymosin Beta 10 (TMSb10) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymosin Beta 10 (TMSb10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Thymosin beta 10 (TMSB10) ELISA试剂盒
    Thymosin beta 10 (TMSB10)
    别名
    Thymosin Beta 10 (TMSB10 产品)
    别名
    TMSB10 ELISA Kit, DKFZp469K1916 ELISA Kit, MIG12 ELISA Kit, TB10 ELISA Kit, Ptmb10 ELISA Kit, THYb10 ELISA Kit, Tb10 ELISA Kit, thymosin beta 10 ELISA Kit, thymosin, beta 10 ELISA Kit, thymosin beta 4, Y-linked ELISA Kit, TMSB10 ELISA Kit, Tmsb10 ELISA Kit, TMSB4Y ELISA Kit
    背景

    Gene Name: Thymosin Beta 10

    Gene Aliases: MIG12, TB10, PTMB10

    途径
    Regulation of Actin Filament Polymerization, Maintenance of Protein Location
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