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Thymic Stromal Lymphopoietin CLIA Kit

This Chemiluminescent ELISA kit is designed for the quantitative measurement of 人 Thymic Stromal Lymphopoietin.
产品编号 ABIN5659203
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Thymic Stromal Lymphopoietin CLIA Kit (ABIN5659203)

抗原

See all Thymic Stromal Lymphopoietin (TSLP) CLIA Kits
Thymic Stromal Lymphopoietin (TSLP)

适用

  • 22
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检测方法

Chemiluminescent

实验类型

Sandwich ELISA

检测范围

7.81 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • 最低检测浓度

    7.81 pg/mL

    Analytical Method

    Quantitative

    特异性

    This assay has high sensitivity and excellent specificity for detection of Thymic Stromal Lymphopoietin (TSLP). No significant cross-reactivity or interference between Thymic Stromal Lymphopoietin (TSLP) and analogues was observed.

    灵敏度

    0.95 pg/mL
  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间

    2 - 3 h

    板类型

    Pre-coated

    实验流程

    The microplate provided in this kit has been pre-coated with an antibody specific to Thymic Stromal Lymphopoietin (TSLP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thymic Stromal Lymphopoietin (TSLP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thymic Stromal Lymphopoietin (TSLP) level in the sample or standard.,

    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymic Stromal Lymphopoietin (TSLP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymic Stromal Lymphopoietin (TSLP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制

    仅限研究用
  • 注意事项

    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    储存条件

    4 °C,-20 °C

    储存方法

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    有效期

    4-8 months
  • 抗原 See all Thymic Stromal Lymphopoietin (TSLP) CLIA Kits

    Thymic Stromal Lymphopoietin (TSLP)

    别名

    Thymic Stromal Lymphopoietin

    背景

    Gene Name: Thymic Stromal Lymphopoietin

    基因ID

    85480

    UniProt

    Q969D9
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