SNCA ELISA 试剂盒
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- 抗原 See all SNCA ELISA试剂盒
- SNCA (Synuclein, alpha (SNCA))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Synuclein Alpha (SNCa). No significant cross-reactivity or interference between Synuclein Alpha (SNCa) and analogues was observed.
- 灵敏度
- 28 pg/mL
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Synuclein, alpha (SNCA) ELISA Kit
SNCA 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synuclein Alpha (SNCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synuclein Alpha (SNCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synuclein Alpha (SNCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synuclein Alpha (SNCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SNCA ELISA试剂盒
- SNCA (Synuclein, alpha (SNCA))
- 别名
- Synuclein Alpha (SNCA 产品)
- 别名
- snca ELISA Kit, MGC64356 ELISA Kit, LOC619283 ELISA Kit, NACP ELISA Kit, alphaSYN ELISA Kit, PARK1 ELISA Kit, PARK4 ELISA Kit, PD1 ELISA Kit, synuclein alpha L homeolog ELISA Kit, alpha-synuclein ELISA Kit, synuclein alpha ELISA Kit, synuclein, alpha ELISA Kit, snca.L ELISA Kit, LOC619283 ELISA Kit, SNCA ELISA Kit, Snca ELISA Kit
- 背景
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Gene Name: Synuclein Alpha
Gene Aliases: SNC-A, aSYN, PD1, PARK1, PARK4, NACP, Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein, Non-A4 component of amyloid precursor
- 基因ID
- 20617
- UniProt
- O55042
- 途径
- Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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