SOD2 ELISA 试剂盒
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- 抗原 See all SOD2 ELISA试剂盒
- SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 2, Mitochondrial (SOD2). No significant cross-reactivity or interference between Superoxide Dismutase 2, Mitochondrial (SOD2) and analogues was observed.
- 灵敏度
- 14.5 pg/mL
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Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit
SOD2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA KitSOD2 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA KitSOD2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA KitSOD2 适用: 兔 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA KitSOD2 适用: 大鼠 Colorimetric Competition ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA KitSOD2 适用: 小鼠 Colorimetric Competition ELISA 0.313-20 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 2, Mitochondrial (SOD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 2, Mitochondrial (SOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 2, Mitochondrial (SOD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 2, Mitochondrial (SOD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SOD2 ELISA试剂盒
- SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))
- 别名
- Superoxide Dismutase 2, Mitochondrial (SOD2 产品)
- 别名
- LOC100101896 ELISA Kit, MNSOD ELISA Kit, GB14346 ELISA Kit, sod2 ELISA Kit, MGC88869 ELISA Kit, Sod2 ELISA Kit, CG8905 ELISA Kit, Dmel\\CG8905 ELISA Kit, Mito SOD ELISA Kit, Mn SOD ELISA Kit, Mn-SOD ELISA Kit, Mn-SOD2 ELISA Kit, MnSOD ELISA Kit, MnSODII ELISA Kit, Mn[2+]SOD ELISA Kit, SOD ELISA Kit, SOD-2 ELISA Kit, SOD2 ELISA Kit, Sod-2 ELISA Kit, dSOD2 ELISA Kit, mitSOD2 ELISA Kit, mnSOD ELISA Kit, IPOB ELISA Kit, MVCD6 ELISA Kit, cb463 ELISA Kit, wu:fj33b01 ELISA Kit, zgc:73051 ELISA Kit, MnSOD ELISA Kit, manganese superoxide dismutase ELISA Kit, superoxide dismutase 2, mitochondrial ELISA Kit, superoxide dismutase 2 ELISA Kit, Mn superoxide dismutase ELISA Kit, Superoxide dismutase 2 (Mn) ELISA Kit, superoxide dismutase 2 L homeolog ELISA Kit, BDBG_07234 ELISA Kit, LOC100101896 ELISA Kit, MNSOD ELISA Kit, Sod2 ELISA Kit, sod2 ELISA Kit, LbMnSOD4 ELISA Kit, SOD2 ELISA Kit, LOC100282741 ELISA Kit, SOD-2 ELISA Kit, sod2.L ELISA Kit
- 背景
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Gene Name: Superoxide Dismutase 2, Mitochondrial
Gene Aliases: IPO-B, MNSOD, Mn-SOD
- UniProt
- P07895
- 途径
- Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling
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