SULF1 CLIA Kit
-
- 抗原 See all SULF1 CLIA Kits
- SULF1 (Sulfatase 1 (SULF1))
-
适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 6.25 pg/mL - 400 pg/mL
- 最低检测浓度
- 6.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Sulfatase 1 (SULF1). No significant cross-reactivity or interference between Sulfatase 1 (SULF1) and analogues was observed.
- 灵敏度
- 0.23 pg/mL
- Featured
- Discover our best selling SULF1 ELISA Kit
- Top Product
- Discover our top product SULF1 ELISA Kit
-
Sulfatase 1 (SULF1) ELISA Kit
SULF1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Sulfatase 1 (SULF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Sulfatase 1 (SULF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Sulfatase 1 (SULF1) level in the sample or standard.,
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfatase 1 (SULF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfatase 1 (SULF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all SULF1 CLIA Kits
- SULF1 (Sulfatase 1 (SULF1))
- 别名
- Sulfatase 1 (SULF1 产品)
- 别名
- HSULF-1 CLIA Kit, SULF-1 CLIA Kit, CG6725 CLIA Kit, DSulf CLIA Kit, DSulf1 CLIA Kit, Dmel\\CG6725 CLIA Kit, Dsulf1 CLIA Kit, Neu5 CLIA Kit, sulf1 CLIA Kit, AI467640 CLIA Kit, AW121680 CLIA Kit, mKIAA1077 CLIA Kit, fk14e08 CLIA Kit, wu:fk14e08 CLIA Kit, SULF1 CLIA Kit, sulf-1 CLIA Kit, XtSulf1 CLIA Kit, hsulf-1 CLIA Kit, XtSulf-1 CLIA Kit, sulfatase 1 CLIA Kit, Sulfated CLIA Kit, extracellular sulfatase SULF-1 homolog CLIA Kit, SULF1 CLIA Kit, Sulf1 CLIA Kit, sulf1 CLIA Kit, LOC410233 CLIA Kit
- 背景
-
Gene Name: Sulfatase 1
Gene Aliases: HSULF-1, Extracellular sulfatase Sulf-1
- 基因ID
- 23213
- UniProt
- Q8IWU6
- 途径
- EGFR Signaling Pathway
-
