BPIFA2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for BPIFA2 ELISA 试剂盒 (ABIN5658776)
抗原
See all BPIFA2 products适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.156 ng/mL
-
原理
- Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) ELISA Kit for detecting SPLUNC2 in serum, plasma, tissue homogenates
-
Analytical Method
- Quantitative
-
特异性
- This assay has high sensitivity and excellent specificity for detection of Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2). No significant cross-reactivity or interference between Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) and analogues was observed.
-
灵敏度
- 0.063 ng/mL
-
组件
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
试剂未包括
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
BPI Fold Containing Family A, Member 2 (BPIFA2) ELISA Kit
BPIFA2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
BPI Fold Containing Family A, Member 2 (BPIFA2) ELISA KitBPIFA2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
BPI Fold Containing Family A, Member 2 (BPIFA2) ELISA KitBPIFA2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
BPI Fold Containing Family A, Member 2 (BPIFA2) ELISA KitBPIFA2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
样本量
- 100 μL
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
-
-
-
注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
有效期
- 12 months
-
-
- BPIFA2 (BPI Fold Containing Family A, Member 2 (BPIFA2))
-
别名
- Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2
-
背景
- Gene Aliases: BPIFA2, PSP, C20orf70, BPI fold-containing family A member 2, Parotid secretory protein
-
基因ID
- 140683
-
UniProt
- Q96DR5
抗原 See all BPIFA2 products
-
