CCL21 CLIA Kit
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北京 101111
Quick Overview for CCL21 CLIA Kit (ABIN5658616)
抗原
See all CCL21 CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 2.74 pg/mL
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原理
- Human Secondary Lymphoid Tissue Chemokine (SLC) CLIA Kit for detecting SLC in serum, plasma
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Secondary Lymphoid Tissue Chemokine (SLC). No significant cross-reactivity or interference between Secondary Lymphoid Tissue Chemokine (SLC) and analogues was observed.
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灵敏度
- 1.28 pg/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA Kit
CCL21 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 人 AA 24-134 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 小鼠 AA 24-133 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 21 (CCL21) ELISA KitCCL21 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Secondary Lymphoid Tissue Chemokine (SLC) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
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别名
- Secondary Lymphoid Tissue Chemokine
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背景
- Gene Aliases: CCL21, 6Ckine, CKb9, ECL, SCYA21, TCA4, ECL, Chemokine (C-C-Motif) Ligand 21, Beta Chemokine Exodus-2, Efficient Chemoattractant For Lymphocytes
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基因ID
- 6366
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UniProt
- Q6ICR7
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途径
- Regulation of Actin Filament Polymerization
抗原 See all CCL21 CLIA Kits
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