Retinoic Acid Receptor alpha ELISA 试剂盒
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- 抗原 See all Retinoic Acid Receptor alpha (RARA) ELISA试剂盒
- Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Retinoic Acid Receptor Alpha (RARa). No significant cross-reactivity or interference between Retinoic Acid Receptor Alpha (RARa) and analogues was observed.
- 灵敏度
- 0.056 ng/mL
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Retinoic Acid Receptor, alpha (RARA) ELISA Kit
RARA 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Retinoic Acid Receptor, alpha (RARA) ELISA KitRARA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor, alpha (RARA) ELISA KitRARA 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Alpha (RARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Alpha (RARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Alpha (RARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoic Acid Receptor Alpha (RARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all Retinoic Acid Receptor alpha (RARA) ELISA试剂盒
- Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))
- 别名
- Retinoic Acid Receptor Alpha (RARA 产品)
- 别名
- NR1B1 ELISA Kit, RAR ELISA Kit, Nr1b1 ELISA Kit, RARalpha1 ELISA Kit, rar-alpha ELISA Kit, etID309833.12 ELISA Kit, rara ELISA Kit, rara2a ELISA Kit, zRAR ELISA Kit, zRAR-alpha ELISA Kit, zgc:109797 ELISA Kit, nr1b1 ELISA Kit, RAR-ALPHA1 ELISA Kit, HS-RARa ELISA Kit, fj66e06 ELISA Kit, rara2b ELISA Kit, wu:fj66e06 ELISA Kit, retinoic acid receptor alpha ELISA Kit, retinoic acid receptor, alpha ELISA Kit, retinoic acid receptor, alpha a ELISA Kit, retinoic acid receptor alpha L homeolog ELISA Kit, retinoic acid receptor, alpha b ELISA Kit, RARA ELISA Kit, Rara ELISA Kit, LOC100136372 ELISA Kit, raraa ELISA Kit, rara.L ELISA Kit, rara ELISA Kit, rarab ELISA Kit
- 背景
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Gene Name: Retinoic Acid Receptor Alpha
Gene Aliases: RAR-A, NR1B1, RAR, RAR-Alpha, Nuclear Receptor Subfamily 1 Group B Member 1
- 基因ID
- 5914
- UniProt
- P10276
- 途径
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins
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