CCL5 CLIA Kit
Our Local Distributor
北京 101111
Quick Overview for CCL5 CLIA Kit (ABIN5658408)
抗原
See all CCL5 CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 27.4 pg/mL
-
原理
- Human Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) CLIA Kit for detecting RANTES in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
-
Analytical Method
- Quantitative
-
特异性
- This assay has high sensitivity and excellent specificity for detection of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). No significant cross-reactivity or interference between Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and analogues was observed.
-
灵敏度
- 11.3 pg/mL
-
组件
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
试剂未包括
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit
CCL5 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA KitCCL5 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
样本量
- 100 μL
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) level in the sample or standard.,
-
实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
-
-
-
注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
有效期
- 12 months
-
-
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
-
别名
- Regulated On Activation In Normal T-Cell Expressed And Secreted
-
背景
- Gene Aliases: CCL5, SCYA5, SISd, EoCP, Chemokine C-C-Motif Ligand 5, Eosinophil Chemotactic Cytokine, T-cell Specific Protein p288, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 5
-
基因ID
- 6352
-
UniProt
- P13501
-
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
抗原 See all CCL5 CLIA Kits
-
