Quinolinic Acid ELISA 试剂盒
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北京 101111
Quick Overview for Quinolinic Acid ELISA 试剂盒 (ABIN5658329)
抗原
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.23 ng/mL
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原理
- Quinolinic Acid (QA) ELISA Kit for detecting QA in serum, plasma
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Quinolinic Acid (QA). No significant cross-reactivity or interference between Quinolinic Acid (QA) and analogues was observed.
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灵敏度
- 0.6 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Quinolinic Acid ELISA Kit
适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum
Quinolinic Acid ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Quinolinic Acid (QA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Quinolinic Acid (QA) and unlabeled Quinolinic Acid (QA) (Standards or samples) with the pre-coated antibody specific to Quinolinic Acid (QA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- Quinolinic Acid
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物质类
- Chemical
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背景
- Gene Aliases: QUIN, Pyridine-2,3-Dicarboxylic Acid
抗原
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