Prothrombin Fragment 1+2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Prothrombin Fragment 1+2 ELISA 试剂盒 (ABIN5658287)
抗原
See all Prothrombin Fragment 1+2 (F1+2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 3.12 ng/mL
-
Analytical Method
- Quantitative
-
特异性
- This assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.
-
灵敏度
- 1.15 ng/mL
-
-
Prothrombin Fragment 1+2 (F1+2) ELISA Kit
F1+2 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prothrombin Fragment 1+2 (F1+2) ELISA KitHigh Sensitivity F1+2 适用: 人 Colorimetric Sandwich ELISA 0.11 pg/mL - 80 pg/mL Plasma, Serum
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: 兔 Colorimetric Sandwich ELISA 0.312 pmol/mL - 20 pmol/mL Plasma, Serum, Tissue Homogenate
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pmol/L - 1000 pmol/L Plasma, Serum, Tissue Homogenate
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: 人 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Prothrombin Fragment 1+2 (F1+2) ELISA KitF1+2 适用: 大鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F1+2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
-
-
-
注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
有效期
- 4-8 months
-
-
- Prothrombin Fragment 1+2 (F1+2)
-
别名
- Prothrombin Fragment 1+2
-
背景
-
Gene Name: Prothrombin Fragment 1+2
-
UniProt
- P18292
抗原 See all Prothrombin Fragment 1+2 (F1+2) ELISA试剂盒
-
