PRSS1 ELISA 试剂盒
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- 抗原 See all PRSS1 ELISA试剂盒
- PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Protease, Serine 1 (PRSS1). No significant cross-reactivity or interference between Protease, Serine 1 (PRSS1) and analogues was observed.
- 灵敏度
- 6.8 pg/mL
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Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA Kit
PRSS1 适用: 鱼 Colorimetric Competition ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA KitPRSS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA KitPRSS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA KitPRSS1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA KitPRSS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA KitPRSS1 适用: 小鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
Protease, serine, 1 (Trypsin 1) (PRSS1) ELISA KitPRSS1 适用: 鱼 Colorimetric Sandwich ELISA 0.312-20 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 1 (PRSS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 1 (PRSS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 1 (PRSS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease, Serine 1 (PRSS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protease, Serine 1 (PRSS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protease, Serine 1 (PRSS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PRSS1 ELISA试剂盒
- PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))
- 别名
- Protease, Serine 1 (PRSS1 产品)
- 别名
- TRP1 ELISA Kit, TRY1 ELISA Kit, TRY4 ELISA Kit, TRYP1 ELISA Kit, LOC397853 ELISA Kit, Trypsin ELISA Kit, wu:fb57g08 ELISA Kit, zgc:109701 ELISA Kit, Dvtry-1 ELISA Kit, dvir_GLEANR_6853 ELISA Kit, DvirGJ21500 ELISA Kit, GJ21500 ELISA Kit, Try1 ELISA Kit, PTRYI ELISA Kit, RATPTRYI ELISA Kit, Try-1 ELISA Kit, Trygn16 ELISA Kit, protease, serine 1 ELISA Kit, protease, serine 1 L homeolog ELISA Kit, trypsin-1 ELISA Kit, Trypsin-1 ELISA Kit, trypsin I ELISA Kit, protease, serine 1 (trypsin 1) ELISA Kit, protease, serine, 1 (trypsin 1) ELISA Kit, PRSS1 ELISA Kit, prss1.L ELISA Kit, prss1 ELISA Kit, CpipJ_CPIJ000708 ELISA Kit, CpipJ_CPIJ007079 ELISA Kit, CpipJ_CPIJ007080 ELISA Kit, CpipJ_CPIJ012156 ELISA Kit, CpipJ_CPIJ016225 ELISA Kit, CpipJ_CPIJ016619 ELISA Kit, CpipJ_CPIJ018339 ELISA Kit, Dvir\Try1 ELISA Kit, trp-i ELISA Kit, Prss1 ELISA Kit
- 背景
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Gene Name: Protease, Serine 1
Gene Aliases: TRP1, TRY1, TRY4, TRYP1, Trypsin 1, Cationic Trypsinogen, Beta-trypsin, Serine protease 1, Trypsin I
- 基因ID
- 5644
- UniProt
- P07477
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