F2RL1 CLIA Kit
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- 抗原 See all F2RL1 CLIA Kits
- F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Protease Activated Receptor 2 (PAR2). No significant cross-reactivity or interference between Protease Activated Receptor 2 (PAR2) and analogues was observed.
- 灵敏度
- 4.9 pg/mL
- Top Product
- Discover our top product F2RL1 ELISA Kit
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Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) ELISA Kit
F2RL1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) ELISA KitF2RL1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) ELISA KitF2RL1 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 nlU/mL - 500 nlU/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) ELISA KitF2RL1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) ELISA KitF2RL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) ELISA KitF2RL1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 2 (PAR2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 2 (PAR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protease Activated Receptor 2 (PAR2) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protease Activated Receptor 2 (PAR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protease Activated Receptor 2 (PAR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all F2RL1 CLIA Kits
- F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))
- 别名
- Protease Activated Receptor 2 (F2RL1 产品)
- 别名
- par2 CLIA Kit, GPR11 CLIA Kit, PAR2 CLIA Kit, Gpcr11 CLIA Kit, PAR-2 CLIA Kit, Par2 CLIA Kit, Par-2 CLIA Kit, Proteinase-activated receptor 2 CLIA Kit, F2R like trypsin receptor 1 CLIA Kit, coagulation factor II (thrombin) receptor-like 1 CLIA Kit, par2 CLIA Kit, F2RL1 CLIA Kit, F2rl1 CLIA Kit
- 背景
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Gene Name: Protease Activated Receptor 2
Gene Aliases: F2RL1, F2-RL1, GPR11, PAR2, Coagulation Factor II(thrombin)receptor-Like 1, G-protein coupled receptor 11, Thrombin receptor-like 1
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
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