PCSK1 ELISA 试剂盒 (Proprotein Convertase Subtilisin/kexin Type 1)

Details for Product PCSK1 ELISA Kit No. ABIN5658128
抗原
  • BMIQ12
  • NEC1
  • PC1
  • PC3
  • SPC3
  • Nec-1
  • Nec1
  • Phpp-1
  • BDP
  • PC1/3
  • proprotein convertase subtilisin/kexin type 1
  • prohormone convertase 1
  • PCSK1
  • Pcsk1
  • pcsk1
  • PC1B
适用
大鼠
其他选择
Kits with alternative reactivity to:
4
2
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
3.12 ng/mL - 200 ng/mL
最低检测浓度
3.12 ng/mL
应用范围
ELISA
选项
样品类型 Plasma, Serum
Analytical Method Quantitative
检测方法 Colorimetric
特异性 This assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) and analogues was observed.
灵敏度 1.44 ng/mL
Plasmids, Primers & others Plasmids, Primers & others PCSK1 products on genomics-online (e.g. as negative or positive controls)
抗原
别名 Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1 ELISA Kit 摘要)
背景

Gene Name: Proprotein Convertase Subtilisin/Kexin Type 1

Gene Aliases: PC1, PC3, SPC3, NEC1, Prohormone Convertase 3, Prohormone Convertase 1, Neuroendocrine Convertase 1, Proprotein Convertase 1

途径 Peptide Hormone Metabolism
说明

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

实验时间 3 h
板类型 Pre-coated
实验流程 The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
实验精密度 Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
限制 仅限研究用
注意事项 The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
储存条件 4 °C,-20 °C
储存方法 -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
有效期 4-8 months
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