PVRL1 ELISA 试剂盒
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- 抗原 See all PVRL1 ELISA试剂盒
- PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Poliovirus Receptor Related Protein 1 (PVRL1). No significant cross-reactivity or interference between Poliovirus Receptor Related Protein 1 (PVRL1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) ELISA Kit
PVRL1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) ELISA KitPVRL1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) ELISA KitPVRL1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor Related Protein 1 (PVRL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poliovirus Receptor Related Protein 1 (PVRL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PVRL1 ELISA试剂盒
- PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
- 别名
- Poliovirus Receptor Related Protein 1 (PVRL1 产品)
- 别名
- CD111 ELISA Kit, CLPED1 ELISA Kit, ED4 ELISA Kit, HIgR ELISA Kit, HVEC ELISA Kit, OFC7 ELISA Kit, PRR ELISA Kit, PRR1 ELISA Kit, PVRR ELISA Kit, PVRR1 ELISA Kit, SK-12 ELISA Kit, nectin-1 ELISA Kit, prr1 ELISA Kit, PVRL1 ELISA Kit, pvrl1 ELISA Kit, AI835281 ELISA Kit, AW549174 ELISA Kit, Cd111 ELISA Kit, HveC ELISA Kit, cd111 ELISA Kit, cd112 ELISA Kit, clped1 ELISA Kit, ed4 ELISA Kit, higr ELISA Kit, hveb ELISA Kit, hvec ELISA Kit, nectin-2 ELISA Kit, ofc7 ELISA Kit, prr ELISA Kit, prr2 ELISA Kit, pvrl2 ELISA Kit, pvrl2.L ELISA Kit, pvrr ELISA Kit, pvrr1 ELISA Kit, pvrr2 ELISA Kit, sk-12 ELISA Kit, nectin cell adhesion molecule 1 ELISA Kit, nectin-1 ELISA Kit, Poliovirus receptor-related protein 1 ELISA Kit, poliovirus receptor-related 1 (herpesvirus entry mediator C) ELISA Kit, nectin cell adhesion molecule 2 L homeolog ELISA Kit, NECTIN1 ELISA Kit, LOC100145553 ELISA Kit, pvrl1 ELISA Kit, nectin1 ELISA Kit, Nectin1 ELISA Kit, PVRL1 ELISA Kit, nectin2.L ELISA Kit
- 背景
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Gene Name: Poliovirus Receptor Related Protein 1
Gene Aliases: CD111, HVEC, PRR1, CLPED1, ED4, HIgR, OFC7, PRR, PVRR1, SK12, Nectin 1, Herpesvirus Entry Mediator C, Herpesvirus Ig-like receptor
- 途径
- Cell-Cell Junction Organization
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