PEX2 ELISA 试剂盒
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- 抗原 See all PEX2 ELISA试剂盒
- PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Peroxisomal Biogenesis Factor 2 (PEX2). No significant cross-reactivity or interference between Peroxisomal Biogenesis Factor 2 (PEX2) and analogues was observed.
- 灵敏度
- 0.056 ng/mL
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Peroxisomal Biogenesis Factor 2 (PEX2) ELISA Kit
PEX2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Peroxisomal Biogenesis Factor 2 (PEX2) ELISA KitPEX2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisomal Biogenesis Factor 2 (PEX2) ELISA KitPEX2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisomal Biogenesis Factor 2 (PEX2) ELISA KitPEX2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisomal Biogenesis Factor 2 (PEX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisomal Biogenesis Factor 2 (PEX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PEX2 ELISA试剂盒
- PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))
- 别名
- Peroxisomal Biogenesis Factor 2 (PEX2 产品)
- 别名
- PAF1 ELISA Kit, PBD5A ELISA Kit, PBD5B ELISA Kit, PMP3 ELISA Kit, PMP35 ELISA Kit, PXMP3 ELISA Kit, RNF72 ELISA Kit, ZWS3 ELISA Kit, D3Ertd138e ELISA Kit, PAF-1 ELISA Kit, Pxmp3 ELISA Kit, pxmp3 ELISA Kit, An18g04150 ELISA Kit, DDBDRAFT_0168812 ELISA Kit, DDBDRAFT_0238045 ELISA Kit, DDB_0168812 ELISA Kit, DDB_0238045 ELISA Kit, peroxisomal biogenesis factor 2 ELISA Kit, RING zinc finger-containing protein ELISA Kit, PEX2 ELISA Kit, Pex2 ELISA Kit, pex2 ELISA Kit, ANI_1_1336164 ELISA Kit, HCAG_04580 ELISA Kit, CC1G_00418 ELISA Kit, PTRG_10616 ELISA Kit, PAAG_05772 ELISA Kit, MGYG_06097 ELISA Kit
- 背景
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Gene Name: Peroxisomal Biogenesis Factor 2
Gene Aliases: PXMP3, PAF1, PMP35, PMP3, RNF72, Peroxin 2, Peroxisome Assembly Factor 1, Peroxisomal Membrane Protein 35 kDa, Peroxisomal Membrane Protein 3
- 基因ID
- 5828
- UniProt
- P28328
- 途径
- Monocarboxylic Acid Catabolic Process
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