PAM ELISA 试剂盒
Quick Overview for PAM ELISA 试剂盒 (ABIN5657690)
抗原
See all PAM ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.25 pg/mL
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Peptidylglycine Alpha Amidating Monooxygenase (PAM). No significant cross-reactivity or interference between Peptidylglycine Alpha Amidating Monooxygenase (PAM) and analogues was observed.
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灵敏度
- 13.5 pg/mL
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Peptidylglycine alpha-Amidating Monooxygenase (PAM) ELISA Kit
PAM 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidylglycine Alpha Amidating Monooxygenase (PAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidylglycine Alpha Amidating Monooxygenase (PAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 4-8 months
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- PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
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别名
- Peptidylglycine Alpha Amidating Monooxygenase
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背景
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Gene Name: Peptidylglycine Alpha Amidating Monooxygenase
Gene Aliases: PAL, PHM, Peptidylamidoglycolate lyase, Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase, Peptidylglycine Alpha-Hydroxylating Monooxygenase
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基因ID
- 5066
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UniProt
- P19021
抗原 See all PAM ELISA试剂盒
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