NUP62 ELISA 试剂盒
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- 抗原 See all NUP62 ELISA试剂盒
- NUP62 (Nucleoporin 62kDa (NUP62))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Nucleoporin 62 kDa (NUP62). No significant cross-reactivity or interference between Nucleoporin 62 kDa (NUP62) and analogues was observed.
- 灵敏度
- 0.112 ng/mL
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Nucleoporin 62kDa (NUP62) ELISA Kit
NUP62 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nucleoporin 62kDa (NUP62) ELISA KitNUP62 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nucleoporin 62kDa (NUP62) ELISA KitNUP62 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleoporin 62 kDa (NUP62). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleoporin 62 kDa (NUP62). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleoporin 62 kDa (NUP62), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleoporin 62 kDa (NUP62) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleoporin 62kDa (NUP62) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleoporin 62kDa (NUP62) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all NUP62 ELISA试剂盒
- NUP62 (Nucleoporin 62kDa (NUP62))
- 别名
- Nucleoporin 62kDa (NUP62 产品)
- 别名
- IBSN ELISA Kit, SNDI ELISA Kit, p62 ELISA Kit, NUP62 ELISA Kit, AA589433 ELISA Kit, AI426861 ELISA Kit, AI790512 ELISA Kit, AU045898 ELISA Kit, D7Ertd649e ELISA Kit, Nupc1 ELISA Kit, CG6251 ELISA Kit, Dmel\\CG6251 ELISA Kit, DDBDRAFT_0167902 ELISA Kit, DDBDRAFT_0237712 ELISA Kit, DDB_0167902 ELISA Kit, DDB_0237712 ELISA Kit, nup62 ELISA Kit, Np62 ELISA Kit, nucleoporin 62 ELISA Kit, hypothetical protein ELISA Kit, nucleoporin 62kDa ELISA Kit, Nucleoporin 62kD ELISA Kit, predicted protein ELISA Kit, nuclear pore protein ELISA Kit, Nup62 nuclear pore complex glycoprotein p62-related protein (IC) ELISA Kit, Nuclear pore glycoprotein p62 ELISA Kit, NUP62 ELISA Kit, PGTG_03077 ELISA Kit, Nup62 ELISA Kit, nup62 ELISA Kit
- 背景
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Gene Name: Nucleoporin 62 kDa
Gene Aliases: IBSN, p62, SNDI, Nuclear Pore Glycoprotein p62
- 基因ID
- 23636
- UniProt
- P37198
- 途径
- EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome
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