电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

NSF ELISA 试剂盒

NSF 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5657148
发货至: 中国
  • 抗原 See all NSF ELISA试剂盒
    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
    适用
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF). No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.
    灵敏度
    0.117 ng/mL
    Featured
    Discover our best selling NSF ELISA Kit
    Top Product
    Discover our top product NSF ELISA Kit
  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all NSF ELISA试剂盒
    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
    别名
    N-Ethylmaleimide Sensitive Factor (NSF 产品)
    别名
    SKD2 ELISA Kit, AI316878 ELISA Kit, AU020090 ELISA Kit, AU067812 ELISA Kit, NSF ELISA Kit, 18.m06598 ELISA Kit, nsf ELISA Kit, si:bz18k17.1 ELISA Kit, wu:fj33g11 ELISA Kit, N-ethylmaleimide sensitive factor ELISA Kit, T1J1.4 ELISA Kit, T1J1_4 ELISA Kit, NEM-sensitive fusion protein ELISA Kit, N-ethylmaleimide sensitive factor, vesicle fusing ATPase ELISA Kit, N-ethylmaleimide sensitive fusion protein ELISA Kit, N-ethylmaleimide-sensitive factor ELISA Kit, N-ethylmaleimide sensitive factor ELISA Kit, N-ethylmaleimide-sensitive fusion protein, putative ELISA Kit, N-ethylmaleimide sensitive factor L homeolog ELISA Kit, vesicle-fusing ATPase ELISA Kit, N-ethylmaleimide-sensitive factor a ELISA Kit, AAA-type ATPase family protein ELISA Kit, NSF ELISA Kit, Nsf ELISA Kit, nsf ELISA Kit, BBOV_II007200 ELISA Kit, TGME49_318510 ELISA Kit, nsf.L ELISA Kit, LOC100180685 ELISA Kit, LOC9310886 ELISA Kit, nsfa ELISA Kit
    背景

    Gene Name: N-Ethylmaleimide Sensitive Factor

    Gene Aliases: SKD2, N-Ethylmaleimide Sensitive Fusion Protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

    基因ID
    4905
    UniProt
    P46459
You are here:
客服