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SMAD1 ELISA 试剂盒

SMAD1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
产品编号 ABIN5656963
发货至: 中国
  • 抗原 See all SMAD1 ELISA试剂盒
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    适用
    • 10
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 1 (Smad1). No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 1 (Smad1) and analogues was observed.
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 1 (Smad1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 1 (Smad1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 (Smad1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 (Smad1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all SMAD1 ELISA试剂盒
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    别名
    Mothers Against Decapentaplegic Homolog 1 (SMAD1 产品)
    别名
    MLP ELISA Kit, Xmad1 ELISA Kit, Xsmad1 ELISA Kit, bsp1 ELISA Kit, jv4-1 ELISA Kit, jv41 ELISA Kit, madh1 ELISA Kit, madr1 ELISA Kit, smad-1 ELISA Kit, MADH1 ELISA Kit, fb39h09 ELISA Kit, wu:fb39h09 ELISA Kit, wu:fb97e04 ELISA Kit, 2/23 ELISA Kit, CG12399 ELISA Kit, Dmel\\CG12399 ELISA Kit, E(zen)2 ELISA Kit, En(vvl) ELISA Kit, MAD ELISA Kit, Mat ELISA Kit, P-Mad ELISA Kit, P-mad ELISA Kit, PMad ELISA Kit, Smad ELISA Kit, Smad1 ELISA Kit, apg ELISA Kit, c28 ELISA Kit, dMAD ELISA Kit, dMad ELISA Kit, l(2)K00237 ELISA Kit, l(2)k00237 ELISA Kit, mad ELISA Kit, p-Mad ELISA Kit, pMAD ELISA Kit, pMad ELISA Kit, pSmad ELISA Kit, BSP-1 ELISA Kit, BSP1 ELISA Kit, JV4-1 ELISA Kit, JV41 ELISA Kit, MADR1 ELISA Kit, AI528653 ELISA Kit, Madh1 ELISA Kit, Madr1 ELISA Kit, Mlp1 ELISA Kit, MusMLP ELISA Kit, smad1 ELISA Kit, xmad ELISA Kit, SMAD family member 1 ELISA Kit, Mothers against dpp ELISA Kit, SMAD family member 1 L homeolog ELISA Kit, smad1 ELISA Kit, SMAD1 ELISA Kit, Mad ELISA Kit, Smad1 ELISA Kit, smad1.L ELISA Kit
    基因ID
    17125
    UniProt
    P70340
    途径
    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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