MT1M ELISA 试剂盒
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北京 101111
Quick Overview for MT1M ELISA 试剂盒 (ABIN5656843)
抗原
See all MT1M ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 2.47 ng/mL
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原理
- Human Metallothionein 1M (MT1M) ELISA Kit for detecting MT1M in serum, plasma
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Metallothionein 1M (MT1M). No significant cross-reactivity or interference between Metallothionein 1M (MT1M) and analogues was observed.
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灵敏度
- 0.97 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Metallothionein 1M (MT1M) ELISA Kit
MT1M 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Metallothionein 1M (MT1M) ELISA KitMT1M 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Metallothionein 1M (MT1M) ELISA KitMT1M 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Metallothionein 1M (MT1M) ELISA KitMT1M 适用: 人 Colorimetric Competition ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Metallothionein 1M (MT1M) ELISA KitMT1M 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Metallothionein 1M (MT1M) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Metallothionein 1M (MT1M) and unlabeled Metallothionein 1M (MT1M) (Standards or samples) with the pre-coated antibody specific to Metallothionein 1M (MT1M). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Metallothionein 1M (MT1M) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Metallothionein 1M (MT1M) in the sample.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metallothionein 1M (MT1M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metallothionein 1M (MT1M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- MT1M (Metallothionein 1M (MT1M))
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别名
- Metallothionein 1M
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背景
- Gene Aliases: MT1K
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基因ID
- 4499
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UniProt
- Q8N339
抗原 See all MT1M ELISA试剂盒
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