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MMP7 CLIA Kit

The 大鼠 MMP7 ELISA Kit (ABIN5656780) is a Chemiluminescent ELISA Kit designed to quantify 大鼠 MMP7.
产品编号 ABIN5656780
发货至: 中国
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Room 801-803
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Quick Overview for MMP7 CLIA Kit (ABIN5656780)

抗原

See all MMP7 CLIA Kits
MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

适用

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大鼠

检测方法

Chemiluminescent

实验类型

Sandwich ELISA

检测范围

156.25 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Plasma, Serum
  • 最低检测浓度

    156.25 pg/mL

    Analytical Method

    Quantitative

    特异性

    This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 7 (MMP7). No significant cross-reactivity or interference between Matrix Metalloproteinase 7 (MMP7) and analogues was observed.

    灵敏度

    6.29 pg/mL
  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间

    2 - 3 h

    板类型

    Pre-coated

    实验流程

    The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 7 (MMP7) level in the sample or standard.,

    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    限制

    仅限研究用
  • 注意事项

    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    储存条件

    4 °C,-20 °C

    储存方法

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    有效期

    4-8 months
  • 抗原 See all MMP7 CLIA Kits

    MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

    别名

    Matrix Metalloproteinase 7

    背景

    Gene Name: Matrix Metalloproteinase 7

    Gene Aliases: MPSL1, MAT, PUMP-1, Matrilysin Uterine, Matrilysin, Matrin, Pump-1 Protease, Uterine Metalloproteinase

    基因ID

    25335

    UniProt

    P50280

    途径

    Production of Molecular Mediator of Immune Response
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