LEFTY1 CLIA Kit
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北京 101111
Quick Overview for LEFTY1 CLIA Kit (ABIN5656345)
抗原
See all LEFTY1 CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.69 pg/mL
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原理
- Human Left/Right Determination Factor 1 (LEFTY1) CLIA Kit for detecting LEFTY1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.
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灵敏度
- 0.25 pg/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Left-Right Determination Factor 1 (LEFTY1) ELISA Kit
LEFTY1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Left-Right Determination Factor 1 (LEFTY1) ELISA KitLEFTY1 适用: 小鼠 Colorimetric Sandwich ELISA 125.00 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Left-Right Determination Factor 1 (LEFTY1) ELISA KitLEFTY1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Left-Right Determination Factor 1 (LEFTY1) ELISA KitLEFTY1 适用: 人 AA 78-366 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
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别名
- Left/Right Determination Factor 1
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背景
- Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B
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基因ID
- 10637
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UniProt
- O75610
抗原 See all LEFTY1 CLIA Kits
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