Lactoferrin ELISA 试剂盒
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- 抗原 See all Lactoferrin (LTF) ELISA试剂盒
- Lactoferrin (LTF) (Lactotransferrin (LTF))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6.25 ng/mL - 400 ng/mL
- 最低检测浓度
- 6.25 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Lactoferrin (LTF). No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.
- 灵敏度
- 2.84 ng/mL
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- Discover our best selling LTF ELISA Kit
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Lactotransferrin (LTF) ELISA Kit
LTF 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Milk, Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Saliva, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Competition ELISA 3.12 μg/mL - 200 μg/mL Milk
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Milk, Plasma, Serum, Urine
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Milk, Plasma, Serum, Urine
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactoferrin (LTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactoferrin (LTF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Lactoferrin (LTF) ELISA试剂盒
- Lactoferrin (LTF) (Lactotransferrin (LTF))
- 别名
- Lactoferrin (LTF 产品)
- 别名
- LTF ELISA Kit, GIG12 ELISA Kit, HLF2 ELISA Kit, LF ELISA Kit, Lf ELISA Kit, Csp82 ELISA Kit, MMS10R ELISA Kit, Ms10r ELISA Kit, PLF ELISA Kit, TFEW ELISA Kit, conalbumin ELISA Kit, lactotransferrin ELISA Kit, transferrin ELISA Kit, transferrin (ovotransferrin) ELISA Kit, LTF ELISA Kit, CpipJ_CPIJ000114 ELISA Kit, TF ELISA Kit, Ltf ELISA Kit
- 背景
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Gene Name: Lactoferrin
Gene Aliases: HLF2, LF, GIG12, Lactotransferrin, Growth-inhibiting protein 12, Talalactoferrin, Lfcin-H, Kaliocin-1
- 基因ID
- 301034
- UniProt
- D3ZAB1
- 途径
- Transition Metal Ion Homeostasis
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