IL-33 CLIA Kit
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- 抗原 See all IL-33 (IL33) CLIA Kits
- IL-33 (IL33) (Interleukin 33 (IL33))
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适用
- 大鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Interleukin 33 (IL33). No significant cross-reactivity or interference between Interleukin 33 (IL33) and analogues was observed.
- 灵敏度
- 0.54 pg/mL
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Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Urine
Interleukin 33 (IL33) ELISA KitIL33 适用: 犬 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 AA 109-266 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 AA 112-270 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 33 (IL33). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 33 (IL33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 33 (IL33) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all IL-33 (IL33) CLIA Kits
- IL-33 (IL33) (Interleukin 33 (IL33))
- 别名
- Interleukin 33 (IL33 产品)
- 别名
- IL33 CLIA Kit, C9orf26 CLIA Kit, DVS27 CLIA Kit, IL1F11 CLIA Kit, NF-HEV CLIA Kit, NFEHEV CLIA Kit, RP11-575C20.2 CLIA Kit, 9230117N10Rik CLIA Kit, Il-33 CLIA Kit, Il1f11 CLIA Kit, RGD1311155 CLIA Kit, interleukin 33 CLIA Kit, IL33 CLIA Kit, Il33 CLIA Kit
- 背景
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Gene Name: Interleukin 33
Gene Aliases: DV27, C9ORF26, IL1F11, NFHEV, Interleukin-1 Family, Member 11, Nuclear factor from high endothelial venules
- 基因ID
- 361749
- UniProt
- Q66H70
- 途径
- Production of Molecular Mediator of Immune Response
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