ITGB1 ELISA 试剂盒
-
- 抗原 See all ITGB1 ELISA试剂盒
- ITGB1 (Integrin beta 1 (ITGB1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Integrin Beta 1 (ITGb1). No significant cross-reactivity or interference between Integrin Beta 1 (ITGb1) and analogues was observed.
- 灵敏度
- 0.28 ng/mL
- Featured
- Discover our best selling ITGB1 ELISA Kit
- Top Product
- Discover our top product ITGB1 ELISA Kit
-
Integrin beta 1 (ITGB1) ELISA Kit
ITGB1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Integrin beta 1 (ITGB1) ELISA KitITGB1 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Integrin beta 1 (ITGB1) ELISA KitITGB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Integrin beta 1 (ITGB1) ELISA KitInstant ITGB1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 1 (ITGb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 1 (ITGb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 1 (ITGb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 1 (ITGb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 1 (ITGb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 1 (ITGb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all ITGB1 ELISA试剂盒
- ITGB1 (Integrin beta 1 (ITGB1))
- 别名
- Integrin Beta 1 (ITGB1 产品)
- 别名
- CD29 ELISA Kit, FNRB ELISA Kit, GPIIA ELISA Kit, MDF2 ELISA Kit, MSK12 ELISA Kit, VLA-BETA ELISA Kit, VLAB ELISA Kit, 4633401G24Rik ELISA Kit, AA409975 ELISA Kit, AA960159 ELISA Kit, ENSMUSG00000051907 ELISA Kit, Fnrb ELISA Kit, Gm9863 ELISA Kit, gpIIa ELISA Kit, cd29 ELISA Kit, fnrb ELISA Kit, gpiia ELISA Kit, itgb1 ELISA Kit, mdf2 ELISA Kit, msk12 ELISA Kit, vla-beta ELISA Kit, vlab ELISA Kit, itgb1-a ELISA Kit, itgb1-b ELISA Kit, integrin subunit beta 1 ELISA Kit, integrin beta 1 (fibronectin receptor beta) ELISA Kit, integrin subunit beta 1 S homeolog ELISA Kit, integrin beta 1 ELISA Kit, integrin subunit beta 1 L homeolog ELISA Kit, ITGB1 ELISA Kit, Itgb1 ELISA Kit, itgb1.S ELISA Kit, LOC100009173 ELISA Kit, itgb1.L ELISA Kit
- 背景
-
Gene Name: Integrin Beta 1
Gene Aliases: CD29, MSK12, ITG-B1, FNRB, GPIIA, MDF2, MSK12, VLAB, Fibronectin Receptor Beta Polypeptide, Antigen CD29 Includes MDF2, Glycoprotein IIa, VLA-4 subunit beta
- 基因ID
- 3688
- UniProt
- P05556
- 途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
-
