Hepcidin ELISA 试剂盒
-
- 抗原 See all Hepcidin (HAMP) ELISA试剂盒
- Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
-
适用
- Pig
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 740.74 pg/mL - 60000 pg/mL
- 最低检测浓度
- 740.74 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc). No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.
- 灵敏度
- 283.51 pg/mL
- Featured
- Discover our best selling HAMP ELISA Kit
- Top Product
- Discover our top product HAMP ELISA Kit
-
Hepcidin Antimicrobial Peptide (HAMP) ELISA Kit
HAMP 适用: 人 Colorimetric Competition ELISA 320 pg/mL - 200000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 大鼠 Colorimetric Competition ELISA 160 pg/mL - 100000 pg/mL Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: Pig Colorimetric Competition ELISA 740.74 pg/mL - 60000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Urine
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHigh Sensitivity HAMP 适用: 大鼠 Colorimetric Sandwich ELISA 246.9 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hepcidin (Hepc) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hepcidin (Hepc) and unlabeled Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to Hepcidin (Hepc). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hepcidin (Hepc) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hepcidin (Hepc) in the sample.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all Hepcidin (HAMP) ELISA试剂盒
- Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
- 别名
- Hepcidin (HAMP 产品)
- 别名
- HEPC ELISA Kit, HFE2B ELISA Kit, LEAP1 ELISA Kit, PLTR ELISA Kit, Hepcidin ELISA Kit, Hamp1 ELISA Kit, Hepc ELISA Kit, Hepc1 ELISA Kit, HAMP ELISA Kit, hepcidin ELISA Kit, hamp1 ELISA Kit, hep1 ELISA Kit, hepcidin antimicrobial peptide ELISA Kit, hepcidin-like ELISA Kit, hepcidin-1 ELISA Kit, Ham percentage ELISA Kit, HAMP ELISA Kit, Hamp ELISA Kit, hamp ELISA Kit, LOC100135935 ELISA Kit, LOC106584587 ELISA Kit
- 背景
-
Gene Name: Hepcidin
Gene Aliases: HAMP, HFE2B, PLTR, LEAP1, Hepcidin Antimicrobial Peptide, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor
- UniProt
- Q8MJ80
- 途径
- Hormone Activity, Transition Metal Ion Homeostasis
-
