HSP90 ELISA 试剂盒
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- HSP90 (Heat Shock Protein 90 (HSP90))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3.12 ng/mL - 200 ng/mL
- 最低检测浓度
- 3.12 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90 (HSP90). No significant cross-reactivity or interference between Heat Shock Protein 90 (HSP90) and analogues was observed.
- 灵敏度
- 1.28 ng/mL
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Heat Shock Protein 90 (HSP90) ELISA Kit
HSP90 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHigh Sensitivity HSP90 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 适用: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90 (HSP90). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90 (HSP90). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90 (HSP90), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90 (HSP90) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90 (HSP90) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90 (HSP90) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all HSP90 ELISA试剂盒
- HSP90 (Heat Shock Protein 90 (HSP90))
- 别名
- Heat Shock Protein 90 (HSP90 产品)
- 别名
- EL52 ELISA Kit, HSP86 ELISA Kit, HSP89A ELISA Kit, HSP90A ELISA Kit, HSP90N ELISA Kit, HSPC1 ELISA Kit, HSPCA ELISA Kit, HSPCAL1 ELISA Kit, HSPCAL4 ELISA Kit, HSPN ELISA Kit, Hsp89 ELISA Kit, Hsp90 ELISA Kit, LAP2 ELISA Kit, git10 ELISA Kit, swo1 ELISA Kit, HSP90 ELISA Kit, htpG ELISA Kit, SCBAC25F8.08 ELISA Kit, 23.m06066 ELISA Kit, 17.m07646 ELISA Kit, HSP90-1 ELISA Kit, 143198_at ELISA Kit, 83 ELISA Kit, 83K HSP ELISA Kit, DMHSP82 ELISA Kit, E(sev)3A ELISA Kit, E(sina)2 ELISA Kit, HSP82 ELISA Kit, HSP83 ELISA Kit, ORF1 ELISA Kit, Su(Raf)3A ELISA Kit, anon-EST:Liang-2.53 ELISA Kit, anon-WO0068693 ELISA Kit, anon-WO0140519.209 ELISA Kit, clone 2.53 ELISA Kit, en(lz)3C/4C ELISA Kit, hsp84 ELISA Kit, l(3)j5C2 ELISA Kit, ms(3)08445 ELISA Kit, stc ELISA Kit, DmelCG1242 ELISA Kit, CG1242 ELISA Kit, Hsp86 ELISA Kit, Hspca ELISA Kit, 86kDa ELISA Kit, 89kDa ELISA Kit, AL024080 ELISA Kit, AL024147 ELISA Kit, Hsp86-1 ELISA Kit, hsp4 ELISA Kit, hsp86 ELISA Kit, hsp89 ELISA Kit, hsp90 ELISA Kit, hsp90a ELISA Kit, hspc1 ELISA Kit, hspca ELISA Kit, hspn ELISA Kit, lap2 ELISA Kit, Hsp90alpha ELISA Kit, heat shock protein 90 alpha family class A member 1 ELISA Kit, heat shock protein Hsp90 ELISA Kit, Hsp90 chaperone ELISA Kit, Heat shock protein 90 ELISA Kit, heat shock protein 90 ELISA Kit, chaperone protein HtpG ELISA Kit, molecular chaperone HtpG ELISA Kit, Heat Shock Protein 90 ELISA Kit, uncharacterized LOC100384473 ELISA Kit, Heat shock protein 83 ELISA Kit, heat shock protein 90, alpha (cytosolic), class A member 1 ELISA Kit, heat shock protein 90 alpha family class B member 1 ELISA Kit, heat shock protein 90kDa alpha family class A member 1 L homeolog ELISA Kit, heat shock protein HSP 90-alpha ELISA Kit, HSP90AA1 ELISA Kit, hsp90 ELISA Kit, HSP90 ELISA Kit, daf-21 ELISA Kit, htpG ELISA Kit, SCO7516 ELISA Kit, MAP_RS10510 ELISA Kit, TP04_0646 ELISA Kit, TP01_0934 ELISA Kit, APH_RS03525 ELISA Kit, GbCGDNIH1_0315 ELISA Kit, MAV_2118 ELISA Kit, HSP90C ELISA Kit, BBOV_IV008400 ELISA Kit, BBOV_III007380 ELISA Kit, ACICU_00312 ELISA Kit, ECL_01244 ELISA Kit, YE105_C1172 ELISA Kit, pco153543(105) ELISA Kit, Hsp83 ELISA Kit, Hsp90aa1 ELISA Kit, HSP90AB1 ELISA Kit, hsp90aa1.1.L ELISA Kit, LOC108698781 ELISA Kit
- 背景
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Gene Name: Heat Shock Protein 90
Gene Aliases: HtpG, Hsp-90 NTD, N-Terminal Domain
- 途径
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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