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Glutathione Reductase ELISA 试剂盒

GSR 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5654702
发货至: 中国
  • 抗原 See all Glutathione Reductase (GSR) ELISA试剂盒
    Glutathione Reductase (GSR)
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78.12 pg/mL - 5000 pg/mL
    最低检测浓度
    78.12 pg/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Glutathione Reductase (GR). No significant cross-reactivity or interference between Glutathione Reductase (GR) and analogues was observed.
    灵敏度
    37 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Reductase (GR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Reductase (GR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Reductase (GR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Reductase (GR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Reductase (GR) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Reductase (GR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Glutathione Reductase (GSR) ELISA试剂盒
    Glutathione Reductase (GSR)
    别名
    Glutathione Reductase (GSR 产品)
    别名
    GR ELISA Kit, ATGR2 ELISA Kit, EMB2360 ELISA Kit, glutathione reductase ELISA Kit, AI325518 ELISA Kit, D8Ertd238e ELISA Kit, Gr-1 ELISA Kit, Gr1 ELISA Kit, DDBDRAFT_0168952 ELISA Kit, DDBDRAFT_0231410 ELISA Kit, DDB_0168952 ELISA Kit, DDB_0231410 ELISA Kit, 2151 ELISA Kit, CG2151 ELISA Kit, DTR ELISA Kit, Dm-TrxR ELISA Kit, DmTR ELISA Kit, DmTrx ELISA Kit, DmTrxR ELISA Kit, DmTrxR-1 ELISA Kit, Dmel\\CG2151 ELISA Kit, Gr ELISA Kit, Trx ELISA Kit, TrxR ELISA Kit, TrxR-1 ELISA Kit, Trxr1 ELISA Kit, anon-WO03040301.185 ELISA Kit, anon-WO03040301.187 ELISA Kit, dTrxR ELISA Kit, dmtrxr-1 ELISA Kit, gr ELISA Kit, l(1)G0154 ELISA Kit, l(1)G0379 ELISA Kit, l(1)G0477 ELISA Kit, l(1)G0481 ELISA Kit, trxr-1 ELISA Kit, gor1 ELISA Kit, glutathione reductase, gro-2 ELISA Kit, glutathione reductase ELISA Kit, glutathione-disulfide reductase ELISA Kit, GR; GRase ELISA Kit, mitochondrial glutathione reductase Pgr1 ELISA Kit, Glutathione reductase (GR) (GRase) ELISA Kit, Thioredoxin reductase-1 ELISA Kit, glutathione-disulfide reductase family protein ELISA Kit, glutathione reductase 1 ELISA Kit, GR ELISA Kit, gor ELISA Kit, GSR ELISA Kit, GSR1 ELISA Kit, Gsr_2 ELISA Kit, Gsr ELISA Kit, GSHR1 ELISA Kit, gsr ELISA Kit, LbGR ELISA Kit, pgr1 ELISA Kit, GSHR2 ELISA Kit, GLR2 ELISA Kit, Bcen_2392 ELISA Kit, RPE_1989 ELISA Kit, HCAG_02219 ELISA Kit, SJAG_01162 ELISA Kit, Trxr-1 ELISA Kit, PF14_0192 ELISA Kit, POPTR_0001s14480g ELISA Kit, gsr1 ELISA Kit
    背景

    Gene Name: Glutathione Reductase

    Gene Aliases: GSR, GLUR, GRD1, Glutathione reductase, mitochondrial

    基因ID
    14782
    UniProt
    P47791
    途径
    Thyroid Hormone Synthesis, Cell RedoxHomeostasis
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