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Follistatin ELISA 试剂盒

FST 适用: 小鼠 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
产品编号 ABIN5654395
发货至: 中国
  • 抗原 See all Follistatin (FST) ELISA试剂盒
    Follistatin (FST)
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    2.47 ng/mL - 200 ng/mL
    最低检测浓度
    2.47 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Follistatin (FS). No significant cross-reactivity or interference between Follistatin (FS) and analogues was observed.
    灵敏度
    0.97 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 h
    板类型
    Pre-coated
    实验流程
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follistatin (FS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follistatin (FS) and unlabeled Follistatin (FS) (Standards or samples) with the pre-coated antibody specific to Follistatin (FS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follistatin (FS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follistatin (FS) in the sample.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin (FS) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin (FS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Follistatin (FST) ELISA试剂盒
    Follistatin (FST)
    别名
    Follistatin (FST 产品)
    别名
    CG12955 ELISA Kit, CG12956 ELISA Kit, CG33466 ELISA Kit, Dmel\\CG33466 ELISA Kit, Fol1 ELISA Kit, dFS ELISA Kit, dFol1 ELISA Kit, dfs ELISA Kit, fs ELISA Kit, FS ELISA Kit, foll ELISA Kit, xfs ELISA Kit, FST ELISA Kit, fst ELISA Kit, AL033346 ELISA Kit, FOL1 ELISA Kit, Fst-288 ELISA Kit, RATFOL1 ELISA Kit, cb446 ELISA Kit, fd07h10 ELISA Kit, fst1 ELISA Kit, wu:fd07h10 ELISA Kit, Follistatin ELISA Kit, follistatin ELISA Kit, follistatin L homeolog ELISA Kit, follistatin a ELISA Kit, Fs ELISA Kit, fst ELISA Kit, FST ELISA Kit, LOC100141662 ELISA Kit, fst.L ELISA Kit, Fst ELISA Kit, fsta ELISA Kit
    背景

    Gene Name: Follistatin

    Gene Aliases: FST, Activin-binding protein

    途径
    Negative Regulation of Hormone Secretion
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