CD147 ELISA 试剂盒
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- 抗原 See all CD147 (BSG) ELISA试剂盒
- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). No significant cross-reactivity or interference between Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) and analogues was observed.
- 灵敏度
- 12.7 pg/mL
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Basigin (Ok Blood Group) (BSG) ELISA Kit
BSG 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Lung Lavage Fluid, Plasma, Serum
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 AA 25-205 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Basigin (Ok Blood Group) (BSG) ELISA KitBSG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CD147 (BSG) ELISA试剂盒
- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
- 别名
- Extracellular Matrix Metalloproteinase Inducer (BSG 产品)
- 别名
- EMMPRIN ELISA Kit, RPE7 ELISA Kit, 5F7 ELISA Kit, CD147 ELISA Kit, M6 ELISA Kit, OK ELISA Kit, TCSF ELISA Kit, AI115436 ELISA Kit, AI325119 ELISA Kit, HT-7 ELISA Kit, 5A11 ELISA Kit, CE9 ELISA Kit, HT7 ELISA Kit, Ox47R ELISA Kit, basignin ELISA Kit, wu:fb26a05 ELISA Kit, wu:fo98e12 ELISA Kit, bsg ELISA Kit, prso03 ELISA Kit, basigin (Ok blood group) ELISA Kit, basigin ELISA Kit, basigin (Ok blood group) S homeolog ELISA Kit, BSG ELISA Kit, Bsg ELISA Kit, bsg ELISA Kit, bsg.S ELISA Kit
- 背景
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Gene Name: Extracellular Matrix Metalloproteinase Inducer
Gene Aliases: CD147, BSG, 5F7, M6, OK, TCSF, Basigin(Ok Blood Group), Collagenase stimulatory factor, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
- 基因ID
- 682
- UniProt
- P35613
- 途径
- S100 Proteins
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